abrB [2017-12-15 18:20:39]

transcriptional regulator of transition state genes

locus: BSU00370

pI: 6.57

mw: 10.63 kDa

protein length: 94 aa

gene length: 282 bp

function: regulation of gene expression during the transition from growth to stationary phase

product: transcriptional regulator

essential: no

synonyms: cpsX,tolB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 44,848 → 45,138

Phenotypes of a mutant

• No swarming motility on B medium [Pubmed|19202088]

The protein

Paralogous protein(s)

• [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh], [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT] (only N-terminal domain)

Modification

• phosphorylated on Ser-86 [Pubmed|20509597] by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC], [protein|E0097A00C82136BB0B1FB89FBA177E28A4EC3D54|PrkD], and [protein|E86A96B832351FF513DD9853EAD8998CC44C9951|YabT] results in loss of DNA-binding activity [Pubmed|24731262]

Effectors of protein activity

• interaction with [protein|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|AbbA] results in inactivation of AbrB [Pubmed|18840696]

Structure

• [PDB|1Z0R] (N-terminal DNA recognition domain), [Pubmed|16223496]
• [PDB|2MJG] (full-length protein) [Pubmed|25308864]

Expression and Regulation

Operons

• genes: [gene|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]
  description: [Pubmed|3145384]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3145384], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, -P [Pubmed|3145384], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|2504584], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • expressed at the onset of stationary phase [Pubmed|3145384]

  additional information

  • degradation of the ''[SW|abrB]'' mRNA is triggered by base-pairing with [SW|RnaC] [Pubmed|25790031]

Biological materials

Mutant

• TT731 (aphA3)
• 1A935 (''abrB''::''cat''), [Pubmed|11886552], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A935&Search=1A935 BGSC]
• GP1548 (''abrB''::''ermC''), available in [SW|Stülke] lab
• BKE00370 (Δ[gene|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTCCCAAGAGATA, downstream forward: _UP4_TAATCATTTCTTGTACAAAA
• BKK00370 (Δ[gene|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTCCCAAGAGATA, downstream forward: _UP4_TAATCATTTCTTGTACAAAA

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

Labs working on this gene/protein

• [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
• [SW|Mark Strauch], Baltimore, USA [http://lifesciences.umaryland.edu/Pages/faculty_profile.aspx?ID=212 homepage]

References

Reviews

• 11964117,19995980,11931565,19202088,18326573,28886686

The [SW|AbrB regulon]:

• 20817675,18840696,23580539

Other original publications

• 19581368,2437099,8878039,11377867,1850083,12586407,2554317,18430133,7768874,2507867,1766371,15687200,1908787,2106683,15687200,2504584,3145384,8821944,8576231,11101897,11395475,11583849,11751836,12123659,12076816,12591885,15610005,16223496,16159768,16702211,17660417,17720793,7592460,19465659,20509597,15101989,19202088,18326573,24534728,24731262,24832089,25002359,25308864,25381239,25790031